Andrew J. Lee: A Window on the Nanoscale: Augmenting High Speed Atomic Force Microscopy with DNA Nanostructures to Study Biomolecular Processes

Andrew J. Lee

University of Leeds, Pollard Institute, School of Electronic and Electrical Engineering

Seminar series of the Institute for Molecular, Cell & Systems Biology

16 January 2020

More about Andrew:   https://eps.leeds.ac.uk/electronic-engineering/staff/785/dr-andrew-j-lee

Biography: 

Dr Andrew Lee is a Research Fellow and has a background in molecular biology, but in his PhD studies moved into bio nanotechnology and microscopy, developing the use of DNA origami structures as frameworks for the AFM analysis of enzyme mechanisms and structures.
His current research interests include:
DNA-based carriers for nanopore biosensor applications, DNA-templated crystallization Reconstruction of biological pathways within DNA reference structures for high-speed AFM analysis, DNA-templated multi-enzyme arrays for enhanced cellulose digestion.

Papers mentioned in the talk:

Rothemund, P. W. K. 2006. Folding DNA to create nanoscale shapes and patterns.
Nature 440: 297–302

Lee AJ, Walti C. 2019. DNA nanostructures: A versatile lab-bench for interrogating biological reactions. Computational and Structural Biotechnology Journal. 832-842 17  

Raveendran M, Lee AJ, Wälti C, Actis P. 2018. Analysis of 2D DNA Origami with Nanopipettes. ChemElectroChem. 3014-3020 5.20  

Lee AJ, Endo M, Hobbs JK, Walti C. 2018. Direct Single-Molecule Observation of Mode and Geometry of RecA-Mediated Homology Search. ACS Nano. 272-278 12.1  

Lee AJ, Sharma R, Hobbs JK, Wälti C. 2017. Cooperative RecA clustering: the key to efficient homology searching. Nucleic Acids Research. 11743-11751 45.20  

Lee AJ, Szymonik M, Hobbs JK, Wälti CP. 2014. Tuning the translational freedom of DNA for high speed AFM. Nano Research. 1811-1821 8.6